Crystalline pyruvate, phosphate dikinase from Bacteroides symbiosus. Modification of essential histidyl residues and bromopyruvate inactivation
نویسندگان
چکیده
منابع مشابه
Crystalline Pyruvate, Phosphate Dikinase from Bacteroides symbiosus
Pyruvate, phosphate dikinase was purified and crystallized from Bacteroides symbiosus. The function of histidyl and cysteinyl residues of the enzyme were investigated by chemical modification with diethylpyrocarbonate and bromopyruvate. Diethylpyrocarbonate rapidly inactivated the enzyme by combination with histidyl residues. The modified enzyme loses the ability to form phosphoryl enzyme and e...
متن کاملBritain Pyruvate , Phosphate Dikinase from Bacteroides symbiosus By RICHARD
1. An improved method is given for preparation of pyruvate,phosphate dikinase from Bacteroidec 8ymbio8sq. 2. The bacterial enzyme is stable, free from interfering enzyme activities, and does not require thiol compounds to maintain stability during storage or assay. 3. New direct assays of enzyme activity are based on acid evolution or consumption as measured at constantpH in a pH-stat. 4. The o...
متن کاملPyruvate, phosphate dikinase of Bacteroides symbiosus. Catalysis of partial reactions and formation of phosphoryl and pyrophosphoryl forms of the enzyme.
Pyruvate, phosphate dikinase from Bacteroides symbiosus catalyzes the conversion of pyruvate, ATP, and Pi to Penolpyruvate, AMP, and PP,. Initial velocity studies by both the forward and reverse reactions in which the three substrates were varied in a constant ratio were found to be in accord with a Tri Uni Uni Ping Pong mechanism. The reaction involves three partial reactions which were invest...
متن کاملThe Pyruvate-Phosphate Dikinase Reaction
was obtained from Bacteroides symbiosus cell extracts and was purified from interfering enzyme activities. It was strongly stimulated by ammonium ion. In this respect and in other physical properties the bacterial enzyme differed from that previously obtained from Entamoeba histolytica. In a complete reaction system the enzyme transferred label from orthophosphate to pyrophosphate and to the y ...
متن کاملIsolation of 3-phosphohistidine from phosphorylated pyruvate, phosphate dikinase.
Pyruvate, phosphate dikinase (EC 2-7-9-1) catalyzes formation of phosphoenolpyruvate, AMP, and inorganic pyrophosphate from pyruvate, ATP, and orthophosphate. A pyrophosphoryl and phosphoryl form of the enzyme is involved in this transfer. The [32P]phosphoryl form of pyruvate, phosphate dikinase was prepared with enzyme isolated from Bacteroides symbiosus. The [32P]phosphoryl enzyme was found t...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1978
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)34420-4